Xiv. an Application of the Method
نویسنده
چکیده
THE method of Hagedorn and Jensen [1923] has become widely used for the estimation of reducing sugar in blood. The method depends upon the fact that potassium ferricyanide is reduced to ferrocyanide when heated in alkaline solution with certain reducing substances. In the Hagedorn and Jensen procedure the ferrocyanide formed in this way is precipitated as the double potassium zinc salt, and the residual ferricyanide is estimated by adding an excess of potassium iodide and acidifying. The ferricyanide is reduced quantitatively by the iodide, and equivalent iodine is liberated according to the equation: 2H3Fe(CN)6 + 2HI = 2H4Fe(CN)6 + I2. The iodine is titrated with thiosulphate. This method based on ferricyanide as the substance reduced has an inherent advantage over copper methods which will be referred to later. In the original form it was devised for estimating the reducing sugar in the filtrates from 041 cc. samples of blood. The capacity is accordingly very small, the upper limit being 0-385 mg. of glucose. Small volumes of solutions are used, and the essential reactants are present in low concentration so that the introduction of impurities may cause relatively large errors. For the purposes of my own work the range of the original method was inconveniently narrow, and the present modification makes possible the estimation of about ten times as much sugar. The new procedure has been standardised for maltose as well as for glucose. No essential change from the original method has been made, except as regards the concentrations and volumes of the various reagents employed. The following solutions are used in the modified method. The chemicals used were of the "Analytical Reagent" quality, as supplied by the British Drug Houses, Ltd., unless otherwise stated.
منابع مشابه
ALKALOIDS OF PAPAVERACEAE (XIV) [I]. ALKALOIDS OF G U UCIUM FIMBRILLIGER UM BOISS ., POPULATION GADUK
Glauciumfimbrilligerum Boiss. population Gaduk of Abbasabad contained three major alkaloids, bulbocapnine (0.5%), isocorydine (0.12%), proptopine (0.2%), and three minor alkaloids, salutaridine (0.01%), N-methyllindcarpine (0.02%) and thaliporphine (0.003%). Bulbocapnine, salutaridine and thaliporphine were detected for the first time in G.fimbril1igeru.m Boiss
متن کاملThe Welfare Cost of Inflation: Theory with an Application of Generalized Method of Moments (GMM) to Ira
متن کامل
An Application of Indirect Sampling Using the Generalized Weight Share Method
This article has no abstract.
متن کاملDevelopment of a novel method for Copper sorption: An application of Taguchi Method
The aim of this study is to investigate the application of polypyrrole/ polyaniline (PPy/PANI) nanofiberfor Cu (II) sorption from paper mill wastewater. The tests and their optimization results were basedon the experiments design in three levels of variables using Taguchi method. The results showed thatin Copper removal tests, the pH of the solution was the most effective para...
متن کاملFlexible web-based management of components for industrial automation
.....................................................................................................................................xiii Zusammenfassung.....................................................................................................................xiv
متن کاملThe plasma membrane carbonic anhydrase in murine hepatocytes identified as isozyme XIV
BACKGROUND Biochemical and histochemical studies have both previously indicated plasma membrane-associated carbonic anhydrase (CA) activity in hepatocytes which has been assumed to be CA IV. However, immunohistochemical data did not support this assignment. Recent northern blotting results indicated the presence of mRNA for the most recently discovered membrane-bound CA isozyme, CA XIV, in the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2005